GeneAbacus 96

Unlock Accelerated Gene Editing Breakthroughs For 96-Well Plate Workflows

Quantify gene-editing efficiency with our PCR-free genotyping reagent kit and obtain immediate actionable results.

GeneAbacus 96 is a standardized genotyping assay that enables rapid, robust, and highly specific gene editing efficiency quantification for large-scale validations. Designed for 96-well plate format and cultured cells or purified DNA as starting material, GeneAbacus 96 accelerates gene editing optimization, target funneling, and iterations with a highly efficient use of time and resources.

The GeneAbacus 96 genotyping kit, includes all probes, reagents, consumables and cloud-based software necessary for quantifying gene editing efficiency.

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 Rapid Turnaround time

Analyze and quantify gene editing efficiency of hundreds of samples per week. Accelerate your funneling timelines.

Single-nucleotide specificity

Achieve single-nucleotide precision and confidence without the need to optimize probes & protocol parameters

 Ready-to-use

All reagents and probes are included in the kit and ready to use. No need for oligo or specific assay design.

Standard Workflow

Same workflow works for all targets. Minimize disruption to established protocols.

Highly Precise Genotyping with GeneAbacus 96

The GeneAbacus 96 workflow begins with purified DNA, cell lines, iPSCs or primary cells subjected to gene editing. Utilizing our innovative, PCR-free chemistry, wild-type and edited genotypes are converted into distinct and countable fluorescent signals that are detected using low magnification fluorescence microscopy or high-content imaging systems. This facilitates the precise differentiation and quantification of genotypes, ensuring exceptional specificity and accuracy in gene editing validation.

Product Specifications

  • 96 reations
  • 50 ng DNA input or cell lines, iPSCs and primary cells
  • 2 day turnaround time
  • Amplicon sequencing level accuracy
  • +/- 10% precision
  • For Human, mouse and zebrafish

Resources

Info Brochure GeneAbacus 96

Poster ESGCT, October 2024

Customer Case Study, July 2024

Collab with SciLifeLab, April 2023

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