A Ready-to-Use Gene Editing Analysis Kit

A customized reagent kit for gene editing efficiency and specificity screening made for researchers in academia and the biotech and pharma industry.

Products

What’s Inside The GeneAbacus?

Countagen’s technology

How It Works

step 1.

Gene Editing Experiment

The gene editing experiment is performed by the customer. Countagen’s technology delivers a customized ready-to-use reagent kit for the analysis of such gene editing experiments.

Step 2.

Cell Lysis & DNA Fragmentation

DNA is extracted from the sample, either cells, tissue or organisms, such as Drosophila.

Step 3.

Specific Probing & Ligation

Tailored padlock probes are used to target the sequences of interest. Only in the event of a perfect match, a DNA ligase seals the nick and locks the padlock probe on the DNA target strand.

Step 4.

Amplification Via RCA

The circularized strands containing the target region are then amplified via Rolling Circle Amplification (RCA). In RCA, the circular template is replicated as a long single-stranded DNA concatemer that spools off when a strand-displacing DNA polymerase amplifies it. The resulting concatemeric amplicon consists of hundreds to thousands of repeats of the circular template.

Step 5.

Labelling

The RCA amplicons, also termed RCA products, are labelled with sequence-specific fluorescent probes. The single-stranded RCA product collapses into a DNA micro-ball and due to the high negative charge stemming from the phosphate backbone it has minimal inter-molecular interactions. This single-molecule integrity makes RCA ideal for digital nucleic acid quantification.

step 6.

Quantification

RCA products can easily be detected using a standard fluorescence microscope and low magnification (10-20X). Countagen’s proprietary technology enriches all RCA products from solution into one field of view, which makes the quantification and analysis extremely simple. As shown in the scheme, RCA products are detected as bright fluorescent dots, where each dot represents a single DNA target from the beginning. This is the strength of RCA, being inherently digital without the need for compartmentalization as in digital PCR.

step 7.

Image Analysis

RCA provides the benefit of being inherently digital as one generated RCA product correspond to a single recognition event. This makes RCA a very powerful technology for digital quantification of nucleic acids with simple image analysis techniques.

As shown in the image on the left hand side, our technology offers in-house analysis of gene editing experiments with single nucleotide precision.

 

Features

Our Benefits

Unique

Inherently digital.
No specialized instrumention.

Ease of Use

Customized plug-and-play solution.

Specific

Highly accurate bioassay chemistry.

Control

Assay stays in the hands of researchers.

Universal

Easily adaptable to other research questions.

Team

A passionate team, ready to assist you.

Get In Touch With Us

Want to try the GeneAbacus in your lab, or still have some questions left how we can simplify and accelerate your gene editing workflow? Don’t hesitate and get in touch with us. We would love to hear from you!