GeneAbacus utilizes the unique technology of padlock probes and rolling circle amplification to speed up and simplify the gene targeting validation process. This assay removes the need for PCR and can be done completely in-house with standard laboratory instrumentation. With GeneAbacus, you are able to bring extracted DNA to quantified data in under 5 hours, with minimal hands-on time and digital quantification precision.

You can request a quote directly on our website (https://countagen.com/quotation-form/) or email info@countagen.com and we will contact you.

GAIA, short for GeneAbacus Image Analyzer, is a light-weight analysis software that quantifies signals and computes editing efficiency.

GAIA is available for macOS and Windows and included in the purchase of GeneAbacus.

There are two boxes that come with the GeneAbacus kit, one box containing the assay components (-20°C) and another box containing the readout components (+4°C storage).

The kits have a 6 months shelf life from date of delivery.

Please store the different components at their recommended temperatures, as specified in the kit documentation and on each box.

The kit is enough for running 32 reactions. We recommend running samples in duplicate for mitigating laboratory errors.

GeneAbacus supports validation of TALENs over CRISPR-based modalities, such as Cas9, Cas12, base and prime editing, to programmable genomic integration (PGI). The GeneAbacus probes precisely target any DNA sequence of interest independently of the editor used.

The protocol consists of around 6 steps, starting with column-extracted genomic DNA, followed by rolling circle amplification, image acquisition and data analysis via GAIA.

Yes, the protocol contains several stopping points to offer flexibility, as noted in the protocol.

There are two types of controls included in the kit. The first is a control of genomic DNA that can be used to confirm correct execution of the protocol. The other control is an internal probe included in the probe mix to validate the amount of genomic DNA and allow for sample normalization.

It is always recommended to include control samples in the experimental design. Besides the edited samples, a wildtype sample has to be included in the analysis. Other controls might include non-sense sgRNA or lack of Cas.

100 ng of DNA (column extracted) is recommended for each reaction. The DNA concentration of the sample should be equal or above 10 ng/µL.

GeneAbacus currently supports column purified DNA from human cell lines and mouse cells, tissue or model organism. The purity of the DNA should be that the 260/280 ratio is >1.7 and 260/230 ratio between 1.8 and 2.2.

For further details or questions, please contact info@countagen.com or via the Contact Us form.

The current version of GeneAbacus requires column purified DNA. Are you in need to run gene edit validation on crude DNA? Get in touch with us at info@countagen.com or via the Contact Us form, and we are happy to discuss.

Yes, the technology is well-suited for high GC content as well as low GC content regions, which are often considered difficult targets for PCR-based assays.

Yes, it is a perfect match for the validation of precision edits as it relies on padlock probe chemistry.

GeneAbacus can quantify editing analysis accurately above 10%.

Single mismatch discrimination accuracy of >95%.

Standard epifluorescence microscopes can be used to read out GeneAbacus. A list of requirements can be found in the table below. Our team is happy to assist you with any questions you may have. Please contact info@countagen.com or via the Contact Us form.