GeneAbacus
Streamlined PCR-free Genotyping
Quantify gene-editing efficiency in just 5 hours with our all-in-one genotyping reagent kit and obtain immediate actionable results.
GeneAbacus: A Standard Genotyping Workflow For Gene Editing Validation
Streamlining Efficiency and Accuracy
GeneAbacus empowers the Cell & Gene Therapy community with a novel genotyping workflow to simplify and accelerate gene editing validation. The GeneAbacus kit, includes probes, reagents, consumables and software necessary for gene editing efficiency quantification. All you need to validate your gene edits within a day.
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A Day Turnaround time
Gain reliable insights into gene editing efficiency and specificity within a single workday. Accelerate your funneling timelines.
Single-Base Precision
Achieve single-nucleotide precision and confidence without the need to optimize probes & protocol parameters
Simple Integration
Compatible with standard laboratory equipment with minimal hands-on time. Minimize disruption to established protocols.
Standard Workflow
Same workflow works for all targets. No need to invest in dedicated optimization efforts.
Beyond the Basics: The Science Behind GeneAbacus
Padlock Probes and Rolling Circle Amplification: Precise and Streamlined Genotyping
Our novel approach — padlock probes and rolling circle amplification — offers single-nucleotide precision and minimal amplification bias.
Starting with column-extracted genomic DNA from a gene editing experiment, our proprietary PCR-free technology converts edited and wild type genotypes into individual fluorescent signals. This enables the precise gene editing validation of cell pools, cell clones and model organisms.
More on how it works here.
GeneAbacus: A Versatile Genotyping Workflow
Knockout
Gene knockouts are often used for for functional analysis of genes. GeneAbacus provides researchers with reliable knockout confirmation results to move swiftly and confidently from gene edit to function analysis.
Knock-in
Validating knock-ins is crucial for precise gene function studies but often challenging due to the complexity of ensuring accurate gene integration. GeneAbacus simplifies this process, enabling researchers to verify their knock-ins within 5 hours.
Base Editing
Base editing allows for precise single-nucleotide changes, critical for gene correction and research. GeneAbacus streamlines the validation process and ensures accurate confirmation of single base edits.
Prime Editing
Prime editing offers precise, versatile gene modifications ranging from small to large edits. GeneAbacus efficiently confirms prime edits to accelerate research journeys and discoveries.
GeneAbacus Empowers Your Discoveries
Faster In-house Analysis
Overcome weeks of downtime waiting for results. Accelerate your journey towards groundbreaking gene-editing discoveries.
Confident Decisions
Gain quick and reliable insights into editing efficiency. Make informed decisions about your gene-editing experiments.
Simplified Workflow
Streamline your workflow with a user-friendly and precise workflow. Free up valuable time and resources.
Product Specifications
| Supported species | Data output | Genomic DNA input | Probe set up | Sample compatibility | Microscope Objective | Turnaround time |
| Human, mouse |
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Custom probes + internal control | Pool analysis or clonal selection | 20x, NA >0.5, WD ~500 µm | 5 hours |
Ready to Experience the GeneAbacus Difference?
Let’s Build Your Future Together
Download Resources
Customer Case Study, July 2024
Collab with SciLifeLab, April 2023
Info Brochure
Scientific Poster